THE LIFE LABORATORY 生命实验室

DATA COLLECTION METHODS

Modern marine biology investigates the ocean across multiple scales, ranging from microorganisms to entire ecosystems. During field expeditions, researchers conduct direct diving observations and utilize specialized equipment to sample seawater, plankton, benthos, and benthic sediments. Where human descent is restricted, robotic complexes and deep-sea submersibles are deployed to bridge the gap.

In the laboratory, samples undergo microscopic examination to identify species, analyze water and soil chemistry, and perform genetic sequencing when required.

A significant portion of oceanographic data is acquired remotely: satellites continuously monitor sea surface temperature, major currents, ice coverage, and phytoplankton blooms. Advanced maps, databases, and computer models then synthesize these results, enabling scientists to uncover global ecological patterns.

In this way, isolated observations coalesce into a comprehensive picture of marine life.

The more diverse the tools of science become, the more secrets are revealed to us!

SEEING THE UNSEEN

How many secrets has the microscope laid bare,
Of unseen particles and veins of body fine and rare!

— Mikhail Lomonosov

The lion’s share of our knowledge regarding the architecture of life has been delivered by the microscope – the primary instrument of the biologist. Microscopy emerged nearly 400 years ago, and the ongoing evolution of this device continues to expand our capacity to visualize the imperceptible.

A fascinating paradox: To discover something entirely new about the World Ocean, sometimes all it takes is peering into a single drop of water!

MICROSCOPY IN MARINE BIOLOGY:

  • Enables precise species identification.
  • Reveals the fine microstructure of organisms and their organ systems.
  • Provides critical insights into the reproduction and early developmental stages of marine animals.
  • Serves as a vital tool for bioindication: the composition and behaviour of microorganisms are analyzed to evaluate water purity, temperature, salinity, and pollution levels.

THE LIGHT MICROSCOPE

  • Magnification: Up to ×1,500
  • Mechanics: The image is generated using visible light and a system of optical glass lenses.
  • Capabilities: Ideal for studying living cells and tissues, allowing researchers to observe biological objects in real-time motion.

THE ELECTRON MICROSCOPE

  • Magnification: Up to ×1,000,000
  • Mechanics: Employs a focused beam of electrons generated by an electron gun, with electromagnetic lenses providing precise beam focusing.
  • Capabilities: Visualizes viruses, cellular membranes, intracellular microstructures, and macromolecular protein complexes.
  • Limitations: Delivers high-resolution detailing at the nanoscale; however, living specimens are generally not examined.

THE UNDERWATER MICROSCOPE

In 2025, researchers at the Scripps Institution of Oceanography developed a breakthrough diver-operated underwater microscope known as the Benthic Underwater Microscope imaging PAM (BUMP). This cutting-edge instrument enables scientists to study the photosynthesis of symbiotic microalgae living directly within coral reef tissues.

MICROSCOPES REVEAL THE COLOURATION OF EXTINCT ANIMALS

Using electron microscopy, scientists search for melanosomes – microscopic cellular organelles that synthesize and store pigments. By analysing their distinct shapes and spatial arrangements, researchers can reconstruct the original colouration of feathers, skin, and scales belonging to ancient creatures.

AGE UNDER THE MICROSCOPE

The age of a fish is determined by examining its scales or otoliths – ‘ear stones’ located within the inner ear of bony fishes. These structures grow incrementally alongside the fish, permanently preserving sequential growth layers. Under a microscope, scientists read these layers much like tree rings to accurately deduce a fish’s age, growth rate, and historical environmental conditions. This method is exceptionally precise for species inhabiting aquatic environments with pronounced seasonality.

A WINDOW INTO THE OCEANIC PAST

Micropaleontology investigates the microscopic, fossilized remains of marine organisms, such as foraminifera, radiolarians, and ostracods. Their preserved shells accumulate within benthic sediments, providing critical data to reconstruct the parameters of ancient seas, historical climate shifts, and the long-term patterns of global ocean currents.

数据采集方法

现代海洋生物学从微生物一直到生态系统,多尺度开展海洋研究。科考中,研究人员开展潜水观测;借助专业设备采集水样、浮游生物、底栖生物、海底沉积物样本。人类难以抵达的区域,依靠机器人、深海潜器完成作业。

实验室环境下,借助显微镜观察样本,鉴定物种,分析水体、底泥组分,必要时开展基因研究。
一部分海洋数据依靠遥感手段获取:卫星观测海水温度、洋流、海冰、浮游植物水华。地图、数据库、计算机模型整合各类观测结果,挖掘内在规律。
一个个独立的观测,拼接成完整的海洋生命图景。
科学工具越丰富,我们揭开的奥秘就越多!
看见看不见的世界
显微镜为我们揭开多少奥秘,
无数微粒,生物体细微的脉络!

— 米哈伊尔・罗蒙诺索夫

显微镜贡献了绝大多数关于生命构造的知识,是生物学家核心工具。显微技术诞生近四百年,仪器不断迭代,拓展人类的可视边界。

有趣的悖论:

想要获知大洋的秘密,有时只需要观察一滴水!

显微镜在海洋生物学中的应用:

  • 鉴定物种
  • 揭示生物机体、器官系统精细结构
  • 研究海洋动物繁殖与早期发育
  • 生物指示:依靠微生物群落组成、状态,判断水体洁净度、温度、盐度与污染情况

光学显微镜

  • 放大倍数 ×1500
  • 依靠可见光与玻璃透镜组成像
  • 可以观察活细胞、活体组织
  • 可以观察动态活体样本

电子显微镜

  • 放大倍数 ×1000000
  • 使用电子束代替光,由电磁透镜实现聚焦
  • 电子枪作为电子源
  • 可以观测病毒、细胞膜、细胞微结构、大型蛋白复合体,实现纳米级细节;一般不能观测活体样本

水下显微镜

2025年,斯克里普斯海洋研究所开发了一种水下显微镜(Benthic Underwater Microscope imaging),可以研究珊瑚礁上共生藻类的光合作用。

显微镜复原灭绝动物体色

科学家借助电子显微镜寻找黑色素体(含色素的微观细胞器),依据它的形态排布,复原古生物羽毛、皮肤、鳞片原本的色彩。

显微镜判定生物年龄

鱼类年龄依靠鳞片,或是硬骨鱼内耳的耳石判断。耳石伴随鱼类生长,留下生长层纹;显微镜下读取层纹,如同树木年轮,判断年龄、生长速度、生存环境。在季节变化明显的水域,这套方法精度极高。

一窥海洋的过去

微体古生物学研究海洋生物的微小化石遗骸(有孔虫、放射虫、介形虫)。它们的外壳保存在海底沉积物中,帮助我们还原古海洋面貌,复原古气候与古洋流变迁。